Open Access Open Badges Research article

Ingestion of 10 grams of whey protein prior to a single bout of resistance exercise does not augment Akt/mTOR pathway signaling compared to carbohydrate

Matthew B Cooke13, Paul La Bounty1, Thomas Buford14, Brian Shelmadine1, Liz Redd1, Geoffrey Hudson15 and Darryn S Willoughby12*

Author Affiliations

1 Department of Health, Human Performance and Recreation, Baylor University, Waco, TX, USA

2 Institute for Biomedical Studies, Baylor University, Waco, TX, USA

3 Schools of Medicine and Human Movement Studies, The University of Queensland, Brisbane, QLD, Australia

4 Department of Aging and Geriatric Research, University of Florida, Gainesville, FL, USA

5 School of Human Performance and Recreation, University of Southern Mississippi, Hattiesburg

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Journal of the International Society of Sports Nutrition 2011, 8:18  doi:10.1186/1550-2783-8-18

Published: 8 November 2011



This study examined the effects of a whey protein supplement in conjunction with an acute bout of lower body resistance exercise, in recreationally-active males, on serum insulin and insulin like growth factor 1 (IGF-1) and Akt/mTOR signaling markers indicative of muscle protein synthesis: insulin receptor substrate 1 (IRS-1), AKT, mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6K) and 4E-binding protein 1 (4E-BP1).


In a randomized, double-blind, cross-over design, 10 males ingested 1 week apart, either 10 g of whey protein (5.25 g EAAs) or carbohydrate (maltodextrose), 30 min prior to a lower-body resistance exercise bout. The resistance exercise bout consisted of 4 sets of 8-10 reps at 80% of the one repetition maximum (RM) on the angled leg press and knee extension exercises. Blood and muscle samples were obtained prior to, and 30 min following supplement ingestion and 15 min and 120 min post-exercise. Serum and muscle data were analyzed using two-way ANOVA.


No significant differences were observed for IGF-1 (p > 0.05). A significant main effect for Test was observed for serum insulin (p < 0.01) at 30 min post-ingestion and 15 and 120 min post-exercise, with no Supplement × Test interaction (p > 0.05). For the Akt/MTOR signaling intermediates, no significant Supplement × Test interactions were observed (p > 0.05). However, significant main effects for Test were observed for phosphorylated concentrations of IRS, mTOR, and p70S6K, as all were elevated at 15 min post-exercise (p < 0.05). Additionally, a significant main effect for Test was noted for 4E-BP1 (p < 0.05), as it was decreased at 15 min post-exercise.


Ingestion of 10 g of whey protein prior to an acute bout of lower body resistance exercise had no significant preferential effect compared to carbohydrate on systemic and cellular signaling markers indicative of muscle protein synthesis in untrained individuals.