Open Access Highly Accessed Research article

Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men; a randomized, double-blinded, placebo-controlled trial

Manfred Lamprecht12*, Simon Bogner12, Gert Schippinger3, Kurt Steinbauer3, Florian Fankhauser3, Seth Hallstroem1, Burkhard Schuetz4 and Joachim F Greilberger15

Author Affiliations

1 Institute of Physiological Chemistry, Centre for Physiological Medicine, Medical University of Graz, Graz, Austria

2 Institute of Nutrient Research and Sport Nutrition, Graz, Austria

3 SportchirurgiePlus, Centre for Individual Sport Medicine and Surgery, Graz, Austria

4 Biovis Diagnostik MVZ GmbH, Limburg, Germany

5 Institute of Laboratory Sciences, Dr Greilberger GmbH, Laßnitzhöhe, Austria

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Journal of the International Society of Sports Nutrition 2012, 9:45  doi:10.1186/1550-2783-9-45

Published: 20 September 2012

Abstract

Background

Probiotics are an upcoming group of nutraceuticals claiming positive effects on athlete’s gut health, redox biology and immunity but there is lack of evidence to support these statements.

Methods

We conducted a randomized, double-blinded, placebo controlled trial to observe effects of probiotic supplementation on markers of intestinal barrier, oxidation and inflammation, at rest and after intense exercise. 23 trained men received multi-species probiotics (1010 CFU/day, Ecologic®Performance or OMNi-BiOTiC®POWER, n = 11) or placebo (n = 12) for 14 weeks and performed an intense cycle ergometry over 90 minutes at baseline and after 14 weeks. Zonulin and α1-antitrypsin were measured from feces to estimate gut leakage at baseline and at the end of treatment. Venous blood was collected at baseline and after 14 weeks, before and immediately post exercise, to determine carbonyl proteins (CP), malondialdehyde (MDA), total oxidation status of lipids (TOS), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). Statistical analysis used multifactorial analysis of variance (ANOVA). Level of significance was set at p < 0.05, a trend at p < 0.1.

Results

Zonulin decreased with supplementation from values slightly above normal into normal ranges (<30 ng/ml) and was significantly lower after 14 weeks with probiotics compared to placebo (p = 0.019). We observed no influence on α1-antitrypsin (p > 0.1). CP increased significantly from pre to post exercise in both groups at baseline and in the placebo group after 14 weeks of treatment (p = 0.006). After 14 weeks, CP concentrations were tendentially lower with probiotics (p = 0.061). TOS was slightly increased above normal in both groups, at baseline and after 14 weeks of treatment. There was no effect of supplementation or exercise on TOS. At baseline, both groups showed considerably higher TNF-α concentrations than normal. After 14 weeks TNF-α was tendentially lower in the supplemented group (p = 0.054). IL-6 increased significantly from pre to post exercise in both groups (p = 0.001), but supplementation had no effect. MDA was not influenced, neither by supplementation nor by exercise.

Conclusions

The probiotic treatment decreased Zonulin in feces, a marker indicating enhanced gut permeability. Moreover, probiotic supplementation beneficially affected TNF-α and exercise induced protein oxidation. These results demonstrate promising benefits for probiotic use in trained men.

Clinical trial registry

http://www.clinicaltrials.gov webcite, identifier: NCT01474629

Keywords:
Probiotics; Leaky gut; Intense exercise; Oxidative stress and inflammation