Table 2

Proteins from myotubes identified by MALDI-TOF MS of spots after 2-DGE.

Spot id

Protein

Sequence coverage^a

Matched peptides^b

Score^c

Theo. pI^d

Theo. Mw^e (kDa)

Access key^f

High in CMH

267

Vimentin

37

21

189

4.9

54

P20152

522

Malate dehydrogenase - cytoplasmic

21

6

65

6.2

37

Q6PAB3

667

Peroxiredoxin-4

26

6

73

6.8

31

O08807

680

Thioredoxin dependent peroxide reductase

45

9

98

5.9

28

P20108

High in Controls

171

GRP75, 75 kDa glucose regulated protein precursor

16

10

76

5.8

74

P38674

941

GRP78, 78 kDa glucose regulated protein precursor

24

16

120

4.9

72

P06761

^a, The minimum coverage of the matched peptides in relation to the full-length sequence.

^b, The number of matched peptides in the database search.

^c, Score of the Mascot search.

^d, Theoretical pI of the full length protein.

^e, Theoretical molecular mass (Mw) of the full length protein.

^f, Primary accession key in the SWISS-PROT database.

Young et al. Journal of the International Society of Sports Nutrition 2010 7:9   doi:10.1186/1550-2783-7-9