Research article

Creatine supplementation spares muscle glycogen during high intensity intermittent exercise in rats

Hamilton Roschel , Bruno Gualano , Marcelo Marquezi , André Costa and Antonio H Lancha Jr

School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil

author email corresponding author email

Journal of the International Society of Sports Nutrition 2010, 7:6doi:10.1186/1550-2783-7-6

Published:	29 January 2010

Abstract

Background

The effects of creatine (CR) supplementation on glycogen content are still debatable. Thus, due to the current lack of clarity, we investigated the effects of CR supplementation on muscle glycogen content after high intensity intermittent exercise in rats.

Methods

First, the animals were submitted to a high intensity intermittent maximal swimming exercise protocol to ensure that CR-supplementation was able to delay fatigue (experiment 1). Then, the CR-mediated glycogen sparing effect was examined using a high intensity intermittent sub-maximal exercise test (fixed number of bouts; six bouts of 30-second duration interspersed by two-minute rest interval) (experiment 2). For both experiments, male Wistar rats were given either CR supplementation or placebo (Pl) for 5 days.

Results

As expected, CR-supplemented animals were able to exercise for a significant higher number of bouts than Pl. Experiment 2 revealed a higher gastrocnemius glycogen content for the CR vs. the Pl group (33.59%). Additionally, CR animals presented lower blood lactate concentrations throughout the intermittent exercise bouts compared to Pl. No difference was found between groups in soleus glycogen content.

Conclusion

The major finding of this study is that CR supplementation was able to spare muscle glycogen during a high intensity intermittent exercise in rats.